Composite

Part:BBa_K2120408

Designed by: Lirong Zhao   Group: iGEM16_BIT-China   (2016-10-13)


B0015+cI+B0034+J23109+pR+B0034+mazF+B0015


<Description>
This is one of our application circuits.


This circuit contains two devices: inhibitor device and killer device.
Inhibitor device: We select weak constitutive promoter J23109 and strong RBS B0034 to express inhibitor gene cI.
Killer device: Toxin protein, MazF, can cleavage ACA sequence of free mRNA specifically. It cause bacteria can’t translate successfully and dead finally. So we constructed it with induced promoter pR and strong RBS B0034 to control the expression level of toxin protein.
We reversed the direction of inhibitor device, in order to reduce the influence between two devices.
Meanwhile, we also constructed more circuits which have different combine with promoter, RBS and inhibitor. More details see in our Wiki([http://2016.igem.org/Team:BIT-China/Parts Parts Submission])


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 941
    Illegal NheI site found at 964
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None